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Relative transcript levels of four candidate EcR-target genes following EcR knockdown in honeybee workers. Twelve pharate-adult workers (Pbl phase) were injected with 5 μg of EcR-dsRNA (KD) or GFP-dsRNA (C, control) and sampled just after adult eclosion. Bars represent means and S.E.M of 12 biological replicates, each run as three technical replicates. *Indicates a statistically significant difference (Mann–Whitney Test, P ≤ 0.05).
