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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Hepatology. 2014 May 27;60(1):237–249. doi: 10.1002/hep.26981

Fig. 3. TLR7 deficiency decreases the induction of type I IFN signaling in DCs.

Fig. 3

(A) mRNA levels of TLR7 was determined by qRT-PCR in isolated liver cell fractions representing HPs, KCs, DCs, and HSCs in WT. (B) mRNA of IFNa4 was determined by qRT-PCR in the WT liver at 30 min after injection of TLR7 ligand (R848). Depletion of DCs was achieved by diphtheria toxin injections (4 ng/g of BW) to CD11c-DTR mice for two consecutive days, and depletion of KCs was done by clodronate liposome injections (200 μl/mouse) for two consecutive days. (C) mRNA levels of IFNa4 and Pin1 were determined by qRT-PCR in isolated DC fraction. The results are shown as fold change compared with normal cells from WT mice. Values presented are from three independent experiments and each sample was assayed in duplicate. (D) Hepatic IFN-α and Pin1 were quantified by Luminex and ELISA, respectively. The results were expressed as ng of IFN-α per mg of liver protein. Data are presented as means ± SEM per group. Different letters indicate significant differences (p < 0.01) according to ANOVA and DMRT (B). Two-tailed Student’s t-test, *P < 0.05, **P < 0.01 (C–D).