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iNOS-KO mice do not compensate by increasing NOS1 or NOS3 expression. C57BL/6J (WT), Nos2tm1Lau, and Nos2 N5 mice were infected with 103 BFT of the Brazil strain of T. cruzi. At 21 dpi, RNAs from cardiac muscle, skeletal muscle, and spleen were extracted, and first-strand cDNA was synthesized. IFN-γ, nNOS, eNOS, and GAPDH transcript levels were quantified by real-time PCR. *, P < 0.05.
