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. 2014 Dec 22;9(12):e114587. doi: 10.1371/journal.pone.0114587

Figure 6. In the absence of Sub1, cells properly exit from exponential phase.

Figure 6

(A) The expression of Pol I- (35S rRNA), Pol II- (RPS6A, IMD2, SSB1, HSP26) or Pol III- (Pre-tRNALeu, pre-tRNAiMet) transcribed genes in the indicated strains grown in SC medium to exponential phase (E) or at day1 was determined by real-time PCR. Expression levels were normalized to those of the 5S rRNA gene and expression levels in the wild type strain grown to exponential phase was set to 100. Means and standard deviations from two independent preparations of cDNA are shown. (B) Samples of the indicated strains grown in SC medium to exponential phase (E) or at day1 were incubated at 55°C for the indicated time (in min) or in the presence of 25 mM H2O2 for 1 h and then spotted onto YPD plates and incubated at 30°C. (C) Changes in DNA content over time in samples of the indicated strains grown in SC medium to exponential phase (E) or at day1 to 4 were determined by flow cytometry. The percentage of viable cells determined by trypan blue staining at each time point is indicated. The percentage of cells in each phase was determined using the cell cycle modelling software ModFit LT 4.0 and is shown in S2 Fig.