Figure 3.

(a) QTL analysis of phorbol ester content based on seeds collected from 120 F₂ plants in mapping population G33 × G43 for linkage group 8. Left y axis: The solid black line shows the F-stat score obtained using GridQTL. The horizontal lines show the genome-wide significance thresholds at P = 0.05 (lower, dashed) and P = 0.01 (upper, dotted) based on 1000 iterations. Right axis: The vertical bars show the QTL distribution calculated using bootstrap analysis (n = 1000). (b) Kernel phorbol ester content of seeds collected from F₂ plants which were homozygous at two markers flanking the seed toxicity locus (‘AA’, G33 allele), heterozygous (‘HH’), or homozygous (‘BB’, G43). The error bars represent the standard deviations.