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. 2004 Jul;72(7):3883–3889. doi: 10.1128/IAI.72.7.3883-3889.2004

FIG. 2.

FIG. 2.

Phylogram of Msp2 and P44 proteins in A. phagocytophilum and A. marginale. The amino acid sequences were aligned with the Protpars program, and the tree was constructed by using the neighbor-joining method of PHYLIP software package 3.6. P44 and Msp2 protein sequences were obtained by BLAST searching the A. phagocytophilum genome, the A. marginale genome, and the GenBank databases using A. phagocytophilum Msp2 (A. phagocytophilum HZ and A. phagocytophilum OS, boldfaced with shaded background). P44 and Msp2 groups are surrounded by dotted lines. Strain names are shown in parentheses. Msp2-SGV1 (AY107766) and Msp2-SGV2 (AY107767) are from A. marginale strain South Idaho (S.ID). Msp2 (AF200927) is from A. marginale strain Idaho7/17 (ID 7/17). Msp2 (AF200925) is from A. marginale strain Florida (FL). Msp2-SGV1, Msp2-SGV2, Msp2-SGV8, Msp2-SGV9, and Msp2-SGV10 (GenBank accession numbers AF290590, AF290591, AF290597, AF290598, and AF290599, respectively) are from A. marginale strain St. Maries (StM). Msp2 proteins from A. marginale strain St. Maries without GenBank accession numbers are referenced by the contig number with the first nucleotide position in the contig (www.vetmed.wsu.edu). P44-2, P44-7, P44-14, P44-18, P44-32, P44-49, and P44-53 are from the HZ genome (www.tigr.org). P44-12 (AF135260 and AY164491) and P44-21 (AF421831 and AY164490) are from strains HZ, NY-18, and Webster (4, 29). P44-20 (AF414591 and AY164492) is from strain HZ and strain HGE2 (4, 30). P44-63 (AY164493) is from HGA patient 2 (P) (4). P44-33 (AY164494) is also from HGA patient 2 (P) (4). Msp1b-StM of A. marginale St. Maries strain is used as an outgroup species.