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. 2014 Dec 22;9(12):e115411. doi: 10.1371/journal.pone.0115411

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A: SDS-PAGE of recombinant protein purified. (Lane M) protein Mark (ordinate values in kDa); (Lane 1) MIC5 protein; B: Immunoblot for the recombinant EaMIC5. (Lane M) protein Mark (ordinate values in kDa); (Lane 1) Recombinant protein MIC5 probed by serum from chickens experimentally infected with E. acervulina as primary antibody; (Lane 2) Recombinant protein MIC5 probed by serum of normal chickens as primary antibody; C: Immunoblot for crude somatic extracts of E. acervulina sporozoites. (Lane M) protein Mark (ordinate values in kDa); (Lane 1) crude somatic extracts of E. acervulina sporozoites probed by serum from rat immunized by EaMIC5. (Lane 2) crude somatic extracts of E. acervulina sporozoites probed by serum of normal rat without immunizing as primary antibody.