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. 2014 Dec 22;9(12):e115165. doi: 10.1371/journal.pone.0115165

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 1 ng Nkx6.3GR mRNA was injected into each cell at 2-cell stage. Animal caps were dissected at Stage 9, treated with cycloheximide for 30 minutes to inhibit protein synthesis, and then cultured in media containing dexamethasone for 2 hours before processed for RT-qPCR to monitor the expression of the marker genes indicated. Among the tested genes, Msx1 was the only one up-regulated clearly under such condition. (B) Dynamic induction of Msx1 by Nkx6.3. The induction of Msx1 was monitored at different time points after induction as described in (A). The expression level of Msx1 was up-regulated in one hour, but was then down-regulated at later stages.