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. 2014 Dec 22;9(12):e115165. doi: 10.1371/journal.pone.0115165

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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RT-PCR analysis in animal cap assay showing the effect of Dlx3 on the expression of the indicated neural crest genes induced by Nkx6.3. Note that the expression of endogenous Dlx3 and Dlx5 were all reduced upon Nkx6.3 over-expression. -RT, negative control with reverse-transcriptase omitted in the RT reaction; embryo, RNA template from whole embryos was used as a positive control.