Figure 2.

Arsenic sulfide-induced apoptosis of gastric cancer cells.

Notes: (A) DAPI staining of AGS and MGC803 cells treated with 1.25 μM As₄S₄ for 0, 24, and 48 hours. (B) Apoptosis analysis by annexin V–FITC/PI double staining of (a) AGS and (b) MGC803 cells treated with As₄S₄ (0 μM, 1.25 μM, 2.5 μM) for 24 hours. Apoptosis ratios represent the percent of cells in the early apoptosis stage (PI-negative/annexin V–FITC-positive). Data represent the mean ± standard deviation of three independent experiments. **P<0.01; ***P<0.001.

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; PI, propidium iodide; Q, quadrant.