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. Author manuscript; available in PMC: 2014 Dec 22.
Published in final edited form as: Nature. 2013 Nov 20;503(7477):548–551. doi: 10.1038/nature12796

Extended Data Figure 5. Inhibitor sensitivity, K-Ras GTP levels and K-Ras dependency of lung cancer cell lines.

Extended Data Figure 5

a, Percentage viability after treatment for 72 h with 12 relative to DMSO (n = 3 biological replicates, error bars denote s.e.m.). b, K-Ras GTP levels determined by incubating lysates with glutathione S-transferase (GST)-tagged RBD (Ras-binding domain of C-Raf) immobilized on glutathione beads (n = 3 biological replicates). c, Viability of cell lines evaluated 72 h after transfection with KRAS siRNA (n = 3 biological replicates). d, K-Ras immunoblot showing knockdown after KRAS siRNA (n = 3 biological replicates).