FIG. 1.

Immunofluorescence patterns of Abs produced in response to C. neoformans in the lung. C. neoformans cells were isolated from mouse lungs after 7 days of infection as described in the text and stained for IgG (A) or IgM (C). (B) Yeast cells from mice given MAb 18B7 and stained for IgG bound to the cells. Four representative fields are shown. (A to C) Light microscopy (left panels) and rhodamine fluorescence patterns (right panels). In panel A, the two bottom panels are high magnifications of a representative cell. An enlargement of the boxed cell in the top right panel is shown in the inset. (D) Cells grown in Sabouraud medium, incubated in serum from infected mice (day 7 of infection), and then assayed for bound IgM (left panel) or IgG (right panel) by using secondary Abs conjugated to FITC or TRITC, respectively. Bars, 10 μm.