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Lack of degradation of NBD lipids after separation on SPE columns. Analysis of NBD-Sph (A) and NBD-C18-ceramide (B) by negative ion mode electrospray tandem mass spectrometry before (upper panel) and after (lower panel) elution from the SPE column. The results were obtained after subtracting peaks obtained upon elution using ethanol-water (1:1, v/v). Intensity refers to ion counts.
