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. 2015 Jan;56(1):22–37. doi: 10.1194/jlr.M051680

Fig. 8.

Fig. 8.

FMO3 overexpression in Tg mice influences liver FMO activity, plasma TMAO, hepatic lipid content, bile acid pool size and composition, body weight, adiposity, and atherosclerotic lesion size. FMO3 Tg/E3L Tg and E3L Tg mice were fed the HF/HC diet for 16 weeks prior to tissue collection. Liver FMO activity (n = 7 for each group) and plasma TMA and TMAO levels (n = 8 to 11 for each group) (A); hepatic levels (n = 10 for each group) of TG, TC, and phosphatidylcholine (PC) (B); and bile acid (BA) pool size and composition (n = 7 to 10 for each group) (C) were measured. For bile acid composition, only the major (≥0.5% of total amount) bile acid species are plotted. Body weight (D) and percent weight (E) of four fat pads (gonadal, retroperitoneal, subcutaneous, and mesentery fat pads), normalized by the body weight, were determined (n = 9 to 12 for each group). F: Mean atherosclerotic lesion sizes at the aortic root region were quantified (n = 15 for E3L Tg, n = 31 for FMO3 Tg/E3L Tg). The lesion data were logarithmically transformed for the t-test. Student’s t-test: * P < 0.05 as compared with the E3L Tg mice.