TABLE 1.
Total numbers and distribution of lymphocytes in submaxillary lymph nodes of STAT6−/−, CD28−/−, and STAT6/CD28−/− mice
| Animalsa | No. of cells/lymph node (106)
|
CD4/CD8 T-cell ratio in lymph nodes | No. of cells/lymph node (106)
|
|||
|---|---|---|---|---|---|---|
| Lymphocytes | T cells | B cells | CD4 T cells | CD8 T cells | ||
| WT | 2.10 ± 0.54b | 1.14 ± 0.24 | 0.59 ± 0.12 | 2.67 ± 0.11 | 0.80 ± 0.20 | 0.29 ± 0.06 |
| STAT6−/− | 1.84 ± 0.25 | 1.0 ± 0.15 | 0.51 ± 0.04 | 3.19 ± 0.26 | 0.78 ± 0.11 | 0.25 ± 0.05 |
| CD28−/− | 1.29 ± 0.51 | 0.72 ± 0.30 | 0.43 ± 0.15 | 2.27 ± 0.32 | 0.46 ± 0.19 | 0.21 ± 0.08 |
| STAT6/CD28−/− (4 mo) | 49.43 ± 0.71c | 22.06 ± 0.02c | 14.36 ± 0.28c | 1.94 ± 0.06 | 15.67 ± 0.89c | 7.99 ± 0.24c |
| STAT6/CD28−/− (8 mo) | 28.52 ± 0.60c | 14.49 ± 0.40c | 10.55 ± 0.50c | 1.63 ± 0.05 | 8.37 ± 0.81c | 5.13 ± 0.38c |
a
WT, STAT6−/−, CD28−/−, and STAT6/CD28−/− BALB/c mice that were 4 and 8 months old (three mice in each group) were killed, and submaxillary lymph nodes were collected. Single-cell suspensions of the lymph nodes were stained to determine the numbers of B cells (B220), T cells (CD3), CD4 T cells (GK1.5), and CD8 T cells (Ly2).
b
Mean ± standard error for three mice.
c
P ≤ 0.01.