Figure 2. Ca²⁺ provoker and buffer expression in NSC-proximal and NSC-distal GIC lines.

Analysis of expression of Ca²⁺ provokers such as one of the permeable glutamate receptor subunits GRIA1 (A) or Ca²⁺ buffer S100A6 (B), ranked the 3 GIC lines (GliNS1 and G166NS, n = 5 each. G179NS, n = 2.) according to Ca²⁺ drug sensitivity, with glutamate channels, such as GRIA1, predicting higher sensitivity and buffer expression predicting lower sensitivity (***p<0.001; Unpaired two-tailed t-test). (C) Western blot analysis showed GRIA1 and S100A6 protein expression, with β-actin as loading control. (D) Protein expression levels of GRIA1 were expressed as percentage fold change when compared against to GliNS1 (n = 3) and (E) S100A6 protein expression levels were expressed as percentage fold change when compared against G166NS (n = 3) (***p<0.001; **p<0.01, *p<0.05 One-way ANOVA).