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. 2014 Dec 22;9(12):e115602. doi: 10.1371/journal.pone.0115602

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© 2014 Cai, Andres

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HA-Rit^Q79L or HA-K-Ras^Q61L containing complex was immunoprecipitated by HA antibody and blotted for Sin1 and Biotin-HA. (B) GFP-Sin1 containing complex was immunoprecipitated by GFP antibody and blotted for HA-Rit^Q79L and Sin1. (C) Lysates from serum-starved WT or Rictor^-/- MEFs overexpressing Flag-EV or Flag-Rit^Q79L were analyzed by immunoblotting with indicated antibodies. (D) Relative phosphorylation of p38 MAPK in WT and Rictor^-/- MEFs overexpressing Flag-EV or Flag-Rit^Q79L. The results are presented as mean ± SEM (One-way ANOVA, n = 3).