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. 2014 Dec 22;9(12):e115602. doi: 10.1371/journal.pone.0115602

Figure 5. mTORC2, but not mTORC1, contributes to cell survival in response to oxidative stress.

Figure 5

(A) Lysates from HeLa cells infected with lentivirus expressing shRNA against either Sin1 or Raptor were analyzed by immunoblotting with indicated antibodies. (Arrowhead, 82 kDa) (B) Cell viability of HeLa-shCTR, HeLa-shSin1 and HeLa-shRaptor was determined by MTS assay 24 h after the initial H2O2 exposure. The results are presented as mean + SEM. (One-way ANOVA followed by Newman-Keuls post-hoc test when appropriate; ** p<0.01, *** p<0.001, n = 3). (C) Cell viability of WT and Rit-/- MEFs was determined by MTS assay 18 h after the initial H2O2 (150 µM) exposure. The results are presented as mean + SEM. (One-way ANOVA followed by Newman-Keuls post-hoc test when appropriate; ** p<0.01, n = 3). (D) Cell viability of HeLa-shCTR, HeLa-shSin1 and HeLa-shRaptor was determined by MTS assay 24 h after the initial ET (20 µM), TM (5 µg/mL) or TG (500 nM) exposure. The results are presented as mean + SEM (One-way ANOVA followed by Newman-Keuls post-hoc test when appropriate; n = 3).