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. 2014 Dec 22;9(12):e115604. doi: 10.1371/journal.pone.0115604

Figure 4. Ventricular wall motion is not fully recovered after cryoinjury.

Figure 4

(A) Schematic representation of segmental criteria of the zebrafish ventricle, considering the antero-posterior and dorso-ventral axis. Depending on their motility, segments are scored as normokinetic (“1”), akinetic (“2”) or dyskinetic (“3”). (B) Theoretical representations of the wall motion score index (WMSI) showing ventricles from healthy controls (WMSI = 1) and cryoinjured animals (WMSI>1). (C) Temporal evolution of changes in the WMSI in cryoinjured animals in a longitudinal study. After injury, the WMSI increased and remained elevated even at 60 dpi, indicating that wall motion is affected. Graphs represent mean values and SD (*** p<0.001; * p<0.05; one-way ANOVA followed by Tukey's honest significant difference test, n = 20). (D) The WMSI is not recovered at extended stages of regeneration (n = 17), and it is not affected in siblings (NI) of the same age (n = 3). *** p = 0.00009, Wilcoxon Signed Rank Test comparing to a theoretical mean of 1. dpi, days postinjury; NI, not injured; WMSI, wall motion score index.