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. Author manuscript; available in PMC: 2014 Dec 22.
Published in final edited form as: Methods Enzymol. 2014;547:373–397. doi: 10.1016/B978-0-12-801415-8.00018-7

Table 18.1.

DNA recognition sequences used to develop specific mito-TALENs to two pathogenic human mtDNA mutations.

TALEN pairs were designed so that cleavage would only occur in the mutated mtDNA molecules.

Target mtDNA recognition sequence
Common deletion (Δ5-mito-TALEN) graphic file with name nihms649102t1.jpg
m.14459G>A (14459A-mito-TALEN) graphic file with name nihms649102t2.jpg

TALEN monomer-binding sites are underlined. In the upper panel, the residual 13-bp repeat unit that flanks deletion breakpoints in full-length mtDNA is in bold. In the lower panel, 14459A is in bold. Numbering is given relative to the rCRS.