TABLE 1.

LTA-induced PCA in human PBMC in the presence or absence of anti-TF IgG or IL-1 Ra

Stimulus	Clotting time (s)a
Medium alone	384 ± 55
Medium + anti-TF IgG	361 ± 110
Medium + control IgG	323 ± 63
Medium + IL-1 Ra	395 ± 55
IL-1β (500 pg/ml)	206 ± 18
IL-1β and IL-1 Ra	400 ± 35***
LTA (1 μg/ml)	127 ± 28
LTA + anti-TF IgG	382 ± 54***
LTA + control IgG	118 ± 43
LTA (0.1 μg/ml)	212 ± 57
LTA + IL-1 Ra	204 ± 53

^aClotting time was determined with a coagulometer. Values represent results from three independent experiments (means ± standard deviations). Statistical analysis was performed by means of ANOVA. No significant differences in clotting times were found in comparisons of the mean for medium alone and the means for controls containing antibodies or IL-1 Ra (overall P value, 0.73). IL-1β in the presence of IL-1 Ra induced a significant increase in clotting time compared with IL-1β alone. LTA (1 μg/ml) in the presence of anti-TF IgG significantly prolonged clotting time compared with LTA alone (overall P value, 0.0046). LTA (0.1 μg/ml) in the presence of IL-1 Ra induced no significant change in clotting time compared with LTA alone (P = 0.053). The level of statistical significance as indicated in the table was set at P of <0.005 (***).