Figure 2. Nucleotides flanking the CpG dinucleotides of DEAF1 half-sites influence DNA binding.

Nucleotide occurrence at the first (A) and second (B) DEAF1 half-sites. Consensus sequences are shown below the matrix for each half-site. EMSA were performed using the indicated 6-space (C) and 8-space (D) ³²P-labeled dsDNA probes. Bold nucleotides indicate the half-site CpG dinucleotides. Underlined nucleotides are mutated in the 6mut and 8mut probes.