Figure 4. DEAF1 binds to 8-spaced TTCG half-sites within the human EIF4G3 promoter.

(A) Schematic of the EIF4G3 promoter showing the location and the reverse complement sequence of the putative DEAF1 response element. Arrow indicates the transcriptional start site. (B) EMSA was performed using the indicated ³²P-labeled dsDNA probe without (-) or with DEAF1 protein (+). (C) ChIP analysis of endogenous DEAF1 interaction with the EIF4G3 promoter. ChIP PCR was performed using the indicated serum and primers that amplified either 249 bp of the promoter region or 298 bp of a region spanning exon 2.