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. 2004 Jul;72(7):4159–4171. doi: 10.1128/IAI.72.7.4159-4171.2004

FIG. 1.

FIG. 1.

SPG-biotin can bind to dectin-1-transduced HEK293 cells. (A and B) Non-dectin-1A-transduced cells (A) and dectin-1-transduced cells (B) were treated with 0, 5, and 100 μg of SPG-biotin per ml. (C) Dose dependence of SPG-biotin binding to dectin-1-transduced cells or control cells, as shown by the fluorescence means from a FACS analysis. Dectin-1-transduced cells were incubated with various concentrations of SPG-biotin for 30 min on ice. After preincubation and washing, the amount of SPG-biotin bound to the cells was determined by staining with streptavidin-Alexa 488 conjugate and using a flow cytometer.