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. 2004 Jul;72(7):4159–4171. doi: 10.1128/IAI.72.7.4159-4171.2004

FIG. 6.

FIG. 6.

Anti-mouse dectin-1 antibody 4B2 recognizes the glucan binding site of dectin 1. The levels of expression of various dectin-1 mutants were monitored by using anti-FLAG MAb. The cells were also assessed to determine their reactivities to dectin-1 MAb 4B2. Dectin-1-transduced cells were incubated with MAb 4B2 for 30 min on ice. After washing, anti-rat IgG-biotin for 4B2 staining and streptavidin-Alexa 488 were added and incubated for 30 min on ice. The cells were analyzed by flow cytometry after antibody staining. The shaded histograms show the results for dectin-1 mutants. The open histograms with the black, blue, and green lines show the results for wild-type dectin-1-transduced cells plus 4B2, control vector-transduced cells plus 4B2, and wild-type dectin-1-transduced cells plus isotype control antibody, respectively.