Table 1.

Kinetic parameters of measured initial substrate concentration ranges ([S]0, μM) of B[a]P and DBC metabolism in the naïve and pregnant B6129SF1/J mouse, male Sprague-Dawley rat and female human. Maximum enzyme velocity (V_max). Michaelis constant (K_m). Michaelis-Menten and In vitro half-life intrinsic clearance rates (CL_INT) scaled to the hepatic tissue and organismal level.

Chemical	Organism	Measured [S] range (μM)	Vmax (nmol/min/mg MSP)	Km (μM)	Tissue level CLINT (ml/min/g tissue)		Organism level CLINT (ml/min/kg body weight)
					MM	t 1/2	MM	t 1/2
B[a]P	Naïve mouse	0.143-20.4	4.35 ± 0.628	4.97 ± 1.96	26.28 ± 14.14	48.53 ± 32.79	1445.2 ± 778.0	2699.0 ± 1803.4
	Male rat	0.161-90.9	1.08 ± 0.401	17.97 ± 12.87	1.8 ± 1.96	1.40 ± 0.61	65.90 ± 71.68	51.34 ± 22.19
	Female human	0.119-2.56	0.025 ± 0.007	0.725 ± 0.581	1.06 ± 1.17	2.13	27.16 ± 29.98	54.69
DBC	Naïve mouse	0.049-9.72	0.646 ± 0.055	6.63 ± 1.01	2.92 ± 0.71	4.59 ± 0.53	160.71 ± 38.94	252.18 ± 29.26
	Pregnant mouse	0.024-8.5	0.417 ± 0.075	3.28 ± 1.38	3.81 ± 2.28	3.31 ± 1.75	159.43 ± 95.51	138.50 ± 72.96
	Male rat	0.107-9.27	0.167 ± 0.064	7.28 ± 4.99	0.69 ± 0.73	1.01 ± 0.30	25.14 ± 26.85	37.05 ± 10.96
	Female human	0.087-1.02	0.009 ± 0.005	0.915 ± 0.823	0.32 ± 0.46	0.16	8.19 ± 11.73	4.23