FIG. 3.

A PI3K inhibitor abolishes P. gingivalis-mediated protection against staurosporine-induced apoptosis. Twenty-four-hour-infected GECs, treated with 20 μM LY294002 (21 h) and then coincubated with 2 μM staurosporine (3 h), are compared with cells with 21 h of infection and 3 h of staurosporine treatment, cells with 24 h of infection only, and uninfected control cells. A significant increase in the amount of cells going through apoptosis is observed for the LY294002-treated cells, as determined by flow cytometry with the Annexin-V-PI assay. Data are expressed as the means from at least two separate experiments, and the values represent the means and standard deviations of separate measurements.