Figure 4.

CUPS formation is specifically triggered by glucose deprivation. (A) Grh1-2×GFP–expressing cells were grown in rich medium, washed, and cultured in 2% potassium acetate, SC-D (−glucose), or SD-N (−nitrogen) media for 0.5 or 2 h and visualized by fluorescence microscopy. (B) Quantification of the phenotypes observed for the distribution of Grh1-2×GFP in A. The data shown are from a single representative experiment out of two repeats. For each condition in the experiment shown, n ≥ 120 cells. (C and D) Cells coexpressing Uso1-2×GFP and Grh1-2×mCherry (C) or GFP-Sed5 and Grh1-2×mCherry (D) were grown in complete medium, then centrifuged and resuspended in SC-D medium to induce glucose deprivation and visualized by fluorescence microscopy at 0.5 and 2 h after incubation in the starvation medium. Broken lines indicate cell boundaries. Bars, 2 µm.