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. 2014 Dec 22;207(6):717–733. doi: 10.1083/jcb.201405077

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© 2014 Klement et al.

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Figure 5. — RNF20 promotes site-specific heterochromatin relaxation in an SNF2H-dependent manner. (A) Schematic for site-specific chromatin relaxation assay. (B) Cells containing an integrated LacO array within a region of heterochromatin were treated ± siRNA toward luciferase (siLuc) or SNF2H (siSNF2H) and transfected with RNF40^Myc and either LacR or LacR-RNF20, as indicated. Color scales indicate false-colored lookup table of pixel intensities (8 bit, pixel range = 1–256). (C and D) The nuclear volume of the LacR-signal (array) as a percentage of the overall nuclear volume was plotted for each condition, either as individual data points (C) or a mean of three independent experiments (D). P-values (standard two-tailed Student’s t test) are indicated. Error bars show SD.