FIG. 1.

Apoptosis of neutrophils (0.5 × 10⁷ to 1 × 10⁷) of osteomyelitis (OM) patients after 12 h of incubation (37°C) in Ham's medium compared to neutrophils of healthy controls. Results from a representative experiment with neutrophils from two patients and two controls are shown in panels A and C. DNA was stained with propidium iodide, and apoptosis was measured by cytometric analysis (A and B) or by agarose gel electrophoresis (C). Left lane in panel C, molecular size markers. In panel B, the 12-h spontaneous apoptosis, in Ham's medium, of 0.5 × 10⁷ to 1 × 10⁷ neutrophils from 52 osteomyelitis patients and 20 controls, measured by cytometric analysis, is represented. Points and bars represent the individual apoptosis values and their means.