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. 2014 Aug 20;29(6):824–836. doi: 10.1177/0885328214548604

Figure 7.

Figure 7.

Histological and immunohistochemical examination of day-28 STRO-1+ SSC explants. STRO-1-immunoselected SSCs from six osteoarthritic individuals (M85, F90, F86, F73, F92, F94) were cultured in Alvetex® 3-D scaffolds for 28 days in chondrogenic media. The overall histology of the cartilaginous explants stained with Alcian blue and Sirius red (A/S) was comparable to that of HAC explants. Chondrogenic differentiation of STRO-1+ SSCs was significantly improved as demonstrated by the robust expression of SOX-9 and collagen Type II, and negligible staining for collagen Type I. Staining for collagen Type X was observed in some cells of the day-28 explants . Scale bars for low and high magnification images represent 200 µm and 50 µm, respectively.