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. Author manuscript; available in PMC: 2015 Jun 5.
Published in final edited form as: Circ Res. 2014 Oct 20;115(12):986–996. doi: 10.1161/CIRCRESAHA.116.303448

Figure 3. Decreased t-tubule density and Amphiphysin II protein levels but unchanged Junctophillin 2 protein levels in ventricular and atrial cells in ovine tachypacing induced heart failure.

Figure 3

A. Representative membrane staining (a), distance maps (b.i.) and mean data summarizing half distances (b.ii.) and t-tubule fractional occupation (b.iii.) from control sheep (left) and heart failure sheep (right) ventricular myocytes. B. Representative membrane staining (a), distance maps (b.i.) and mean data summarizing half distances (b.ii.) and t-tubule fractional occupation (b.iii.) from control sheep (left) and heart failure sheep (right) atrial myocytes. Data from (cells/hearts); Ventricle, 67/6 control, 56/7 heart failure; Atria, 21/5 control, 18/2 heart failure. C. Representative Western blots (upper panels) and summary data (lower panels) showing changes in AmpII protein levels in ventricular (a) and atrial (b) tissues. D. Representative Western blots (upper panels) and summary data (lower panels) showing JPH2 protein levels in ventricular (a) and atrial (b) tissues. IC, internal control; *, P < 0.05; **, P < 0.01; ***, P < 0.001. For Western blots data are normalized to the respective internal control sample used in each experiment. For Western blotting N = 7 control ventricle, 7 heart failure ventricle, 7 control atria, 6 heart failure atria.