Table 1.
A comparison of genetic changes found at sites of double-strand break repair resulting from genetic engineering (Forsbach et al. 2003), cleavage with a restriction endonuclease (Salomon and Puchta 1998) and mutagenesis with gamma irradiation (Morita et al. 2009)
| Microhomology | Insertions | Deletions | Rearrangements | |||||
|---|---|---|---|---|---|---|---|---|
| Number of sites/total sites analyzed | Size range (bp) | Number of sites/total sites analyzed | Size range (bp) | Number of sites/total sites analyzed | Size range (bp) | Number of sites/total sites analyzed | Size range (bp) | |
| Forsbach et al. (2003) | 66/186a | 1–8 | 109/186a | 1–1,700 | 64/73 | 1–300 | 3/73 | 20, translocations |
| Salomon and Puchta (1998) | 13/17 | 1–15 | 18/28 | 4–2,355 | 27/28 | 1–1,322 | 0/28 | N/A |
| Morita et al. (2009) | 16/21 | 1–8 | 1/21 | 1 | 21/21 | 1–129,700 | 2/21 | 1,284,800; 3,208,500 |
The number of sites at which microhomologies, insertions, deletions and rearrangements were identified is shown versus the total number of sites analyzed, as well as the size range of each
aSequences at the left and right borders of the T-DNA insertions were analyzed separately