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. 2014 Sep 29;143(1):36–45. doi: 10.1093/toxsci/kfu203

FIG. 4.

FIG. 4.

ARNT overexpression rescues milk production in the presence of AHR agonists. A, A HA-tagged ARNT construct (pEiZ-HA-ARNT) was stably expressed in mammary HC11 cells using lentiviral transduction. An empty expression vector (pEiZ) was used to create a stable control HC11 cell line. Western blot of lysates probed for HA and α-tubulin (control). B, Stable HC11 cell lines were treated with vehicle control (DMSO), 10 μM 1023 or 10 nM TCDD and induced with lactogenic hormones (LH) to produce milk proteins. Western blot of lysates probed for β-casein and α-tubulin (control). Abbreviations: HA, hemagglutinin; ARNT, aryl hydrocarbon receptor nuclear translocator; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; HA, hemagglutinin; AHR, aryl hydrocarbon receptor; DMSO, dimethyl sulfoxide.