FIG. 11.

Downregulation of 4E-BP1 partially attenuates rotenone-induced H₂O₂ and apoptosis in neuronal cells. PC12 cells and primary neurons, infected with lentiviral shRNA to 4E-BP1 or GFP (as control), were exposed to rotenone (0.5 and/or 1 µM) for 24 h, followed by (A and B) Western blotting using the indicated antibodies, (C) cell viability evaluation using the MTS assay, (D) cell apoptosis analysis using DAPI staining, or (E) H₂O₂ imaging using a peroxide-selective probe H₂DCFDA. For (A) and (B), the blots were probed for β-tubulin as a loading control. Similar results were observed in at least three independent experiments. For (C), (D), and (E), all data were expressed as means ± SEM (n = 6). ^ap < .05, difference with control group; ^bp < .05, 4E-BP1 shRNA group versus GFP shRNA group.