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Rotenone induces activation of caspase cascade in neuronal cells. The indicated cells were treated with rotenone (0–1 μM) for 24 h (A), or rotenone (1 μM) for 0–24 h (B), followed by Western blotting using the indicated antibodies. Blots were probed for β-tubulin as a loading control. Similar results were observed in at least three independent experiments.
