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Rotenone induces ROS production in neuronal cells. The indicated cells were treated with (A) rotenone (0–1 μM) for 24 h, or (B) rotenone (1 μM) for 0–24 h, followed by ROS assay using an oxidant-sensitive probe CM-H2DCFDA. All data were expressed as means ± SEM (n = 6). *p < .05, **p < .01, difference with control group.
