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. 2014 Dec 12;46(12):e125. doi: 10.1038/emm.2014.77

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Copyright © 2014 KSBMB.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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The accumulation of YAP/TAZ in the subcellular fractionation to generate cytoplasmic and nuclear fractions. The sample was fractionated to generate cytoplasmic and nuclear fractions in control conditions or after treatment for 2 h with 1 μM LPA. The presence of the cytoplasmic marker β-tubulin and the nuclear marker lamin A/C in the appropriate fraction was confirmed by western blotting with the corresponding antibodies. The results are representative of three experiments. LPA, lysophosphatidic acid; TAZ, transcriptional co-activator with a PDZ-binding motif; YAP, Yes-associated protein.