TABLE 2.

PCR primers

Primer	Restriction site	Sequencea
Primers used for allelic gene replacement
ermR2	SacI	5′-CTC GAG CTC TGA CGG TGA CAT CTC TCT ATT G-3′
ermL1	NheI	5′-CTC GCT AGC GAA AAG TAC CAT AAA CGG TCG-3′
vrsbUR1	BamHI	5′-CTC GGA TCC AGC GAA AAT ACC AAC CCA CG-3′
vrsbUL1	SacI	5′-CTC GAG CTC GAA ATG CGC CTC CTT ACT TC-3′
hrsbUR1	NheI	5′-CTC GCT AGC GAT GGT GTT ACA GAG GCA CG-3′
hrsbUL1	KpnI	5′-CTC GGT ACC AGC TGG CAA CCG CAT TTC-3′
hrsbWR1	NheI	5′-CTC GCT AGC GTT ATT TCA GAC CAA GGT G-3′
hrsbWL1	KpnI	5′-CTC GGT ACC TTA TCA TTC TGT TGT CCC AT-3′
vsigBR1	BamHI	5′-CTC GGA TCC CCA ATG AGA CAA GAA GGC AC-3′
vsigBL1	SacI	5′-CTC GAG CTC CTT GAG CTT GGC TAT CTT CG-3′
hsigBR1	NheI	5′-CTC GCT AGC CGA AAG AAG CTC AGG TGG AC-3′
hsigBL1	KpnI	5′-CTC GGT ACC GAT GCT GAA TAA ACT GAT GCG-3′
Primers used for Northern blot hybridization probes
erm.for	None	5′-AAT TGG AAC AGG TAA AGG GC-3′
erm.rev	None	5′-AAC ATC TTG GGT ATG GCG G-3′
sigB.for	None	5′-AGA TTT AGT TCA AGT TGG TA-3′
sigB.rev	None	5′-TTA TCA TCT TGT TGT CCC AT-3′
rsbU.for	None	5′-GAA GTG GAA GTA AGG AGG CG-3′
rsbU.rev	None	5′-TCG ATG TGT TAC CAG AAG TCG-3′
sarA.for	None	5′-ATA GGG AGG TTT CAT TAA TGG C-3′
sarA.rev	None	5′-TTT GCT TCT GTG ATA CGG TTG-3′
asp23.for	None	5′-AAA ATC AAA AAG CAC TTG AGC G-3′
asp23.rev	None	5′-AAA AAA TTG CAG GTAT TGC AG-3′
icaA.for	None	5′-GAA TCC AAA ATT AGG CGC AG-3′
icaA.rev	None	5′-AAC ATC CAG CAT AGA GCA CG-3′
icaR.for	None	5′-TCC GAA AAG GGG TAC GAT G-3′
icaR.rev	None	5′-CCT CTT TAT CCA AAG CGA TG-3′
Primers used for quantitative RT-PCR
asp23.real1	None	5′-TCC AAC TTC TAC AGA TAC GCC-3′
asp23.real2	None	5′-AAA ATT GCA GGT ATT GCA GC-3′
icaA.real1	None	5′-TGT ATC AAG CGA AGT CAA TCT C-3′
icaA.real2	None	5′-GGC ACT AAC ATC CAG CAT AG-3′
icaR.real1	None	5′-TGA AGA TGG TGT TTG ATT TGT G-3′
icaR.real2	None	5′-CCA TTG ACG GAC TTT ACC AG-3′
rsbU.real1	None	5′-AGC GTT TGA GGA AAT TGG TGT G-3′
rsbU.real2	None	5′-CCT CTA CAT CTC GTG CCT CTG-3′
gyrB.real1	None	5′-CTG ACA ATG GCC GTG GTA TTC-3′
gyrB.real2	None	5′-GAA GAT CCA ACA CCG TGA AGA C-3′

^aRestriction sites are underlined.