Table 2. Differentially expressed genes of S. wittichii RW1 common to pair-wise comparisons in short contact sand experiments.
Gene | Annotation |
Fold change in comparisona |
|||||
---|---|---|---|---|---|---|---|
SAL-NOTHb vs ctrl SAL | SAL-DBF vs ctrl SAL | DBF-DBF vs ctrl DBF |
Two-way ANOVAc |
||||
1 h | EXPO | STAT | |||||
Swit_0061 | Ribosomal large subunit pseudouridine synthase D | −5.3 | −3.3 | 2 | 2.6 | −1.9 | 1.0 |
Swit_0266 | UspA domain-containing protein | −4.7 | −5.7 | −4.6 | −1.7 | −3.2 | −1.1 |
Swit_0540 | TonB-dependent receptor | −2.6 | −2.3 | 3.5 | 2.6 | −1.4 | 1.1 |
Swit_0652 | Methylmalonate-semialdehyde dehydrogenase | 4.2 | 4.5 | 6.5 | 1.1 | 6.2 | 1.9 |
Swit_0975 | Muconate cycloisomerase | −21.7 | −21.1 | −6.3 | 1.5 | −1.9 | −1.1 |
Swit_0976 | Muconolactone Delta-isomerase | −12.9 | −11.9 | −4.5 | 1.7 | −5.4 | 1.2 |
Swit_0977 | Catechol 1,2-dioxygenase | −11.4 | −12.9 | −9.8 | −1.1 | −2.6 | 1.6 |
Swit_1124 | Glutaredoxin | 3.7 | 4.3 | 2.7 | 1.8 | 2.2 | 33.9 |
Swit_1200 | Globin | −21.8 | −22.1 | −3.3 | −4.6 | −1.3 | −1.3 |
Swit_1327 | 30S ribosomal protein S11 | −2.3 | 2.5 | 10.5 | 2.2 | 1.4 | 2.6 |
Swit_1328 | 30S ribosomal protein S13 | −2.1 | 2.8 | 14.1 | 4.9 | 1.5 | 2.4 |
Swit_1336 | 30S ribosomal protein S5 | −6.3 | −2.3 | 35.2 | 7.7 | −1.4 | 1.2 |
Swit_1346 | 50S ribosomal protein L16 | −5.6 | −2.2 | 22.2 | 4.6 | −1.3 | −1.0 |
Swit_1799 | Cbb3-type cytochrome oxidase component | −4.4 | −4.5 | −3.5 | −1.5 | −2.8 | −1.7 |
Swit_1800 | Cytochrome c oxidase, cbb3-type, subunit II | −3.3 | −3.9 | −2.8 | 1.2 | −2.4 | −1.4 |
Swit_2401 | 5,10-Methylenetetrahydrofolate reductase | −14.3 | −2.4 | 4.6 | 3.0 | −3.1 | −1.5 |
Swit_2914 | FeS assembly SUF system protein | −6.2 | −3.1 | 3.7 | 2.3 | −1.1 | 1.4 |
Swit_3044 | TonB-dependent receptor | −3.1 | −8.8 | −4.6 | 2.7 | −4.0 | −18.7 |
Swit_3144 | TonB-dependent receptor | −3.1 | −8.2 | −17.8 | −1.0 | −1.4 | 3.4 |
Swit_3256 | TonB-dependent receptor | −2.5 | −8.2 | −6.5 | 3.7 | −1.6 | 1.6 |
Swit_3587 | Alkyl hydroperoxide reductase | 2.5 | 6.3 | 5.4 | 49.3 | −1.1 | 7.4 |
Swit_3729 | Hypothetical protein Swit_3729 | −3 | −3.9 | −8.2 | −1.2 | −1.8 | 5.3 |
Swit_3904 | Hypothetical protein Swit_3904 | 11.7 | 9.8 | 3.8 | 51.7 | 2.4 | 31.3 |
Swit_3960 | Hypothetical protein Swit_3960 | 2.5 | 4.1 | 4.4 | 1.7 | 1.6 | 5.0 |
Swit_4364 | Methylamine dehydrogenase accessory protein MauD | 9 | −2.2 | −3.1 | 1.6 | −1.1 | −2.5 |
Swit_4662 | BLUF domain-containing protein | −2.8 | −7.9 | −9.2 | 1.0 | −3.1 | 1.8 |
Swit_5202 | Hypothetical protein Swit_5202 | −8.7 | −5.6 | −2.6 | 1.2 | 1.4 | −2.5 |
Abbreviation: ANOVA, analysis of variance.
Fold change (absolute) in condition compared with control, negative values indicate decreased expression in soil.
Sample indications: Bare sand vs control cells growing in salicylate (SAL-NOTH vs ctrl SAL), sand contaminated with DBF vs control cells growing in salicylate (SAL-DBF vs ctrl SAL), and sand contaminated with DBF vs control cells growing in dibenzofuran (DBF-DBF vs ctrl DBF).
Two-way ANOVA in the condition ‘growth phase' 1 h: DBF-DBF vs ctrl DBF, EXPO: SAND-DBF-EXPO vs wsDBF, and STAT: SAND-DBF-STAT vs LIQ-DBF-STAT.