FIG. 8.

Quantitative analysis of the integrity of LCPs in macrophages (A) and A. polyphaga (B) at 12, 18, and 24 h postinfection. The percentages of LCPs positive for acid phosphatase were evaluated when the bacteria were within an intact phagosome (IP), a disrupted phagosome (DP), and in the cytoplasm (Cyt). Averages of 60, 50, and 10 to 30 LCPs were analyzed for both macrophages and A. polyphaga at 12, 18, and 24 h, respectively. At 24 h, the number of LCPs analyzed was low because most of the cells had been lysed. Error bars represent standard deviations.