TABLE 1.

Primer sequences and cycling conditions^a

Gene product	Primer sequence (5′→3′)	Tm (°C)	Product (bp)
S100A8	(F)/ATTTCCATGCCGTCTACAGG	60	247
	(R)/CAGCCTCTGGGCAGTAACTC
S100A9	(F)/CAGCTGGAACGCAACATAGA	60	229
	(R)/TCAGCATGATGAACTCCTCG
S100A10	(F)/AAATTCGCTGGGGATAAAGG	60	289
	(R)/TCTTATCAGGGAGGAGCGAA
S100A12	(F)/CTTGAAGAGCATCTGGAGGG	60	328
	(R)/CTCATTGAGGACATTGCTGG
S100A13	(F)/AGCTAGCCCCTTGAGGACAT	60	268
	(R)/TTGAGCTCCGAGTCCTGATT
TNF-α	(F)/AAGAATTCAAACTGGGGCCT	61	432
	(R)/GGCTACATGGGAACAGCCTA
IL-1β	(F)/TCCAGGGACAGGATATGGAG	61	308
	(R)/TTCTGCTTGAGAGGTGCTGA
IL-8	(F)/TAGCAAAATTGAGGCCAAGG	61	214
	(R)/GGACTTGTGGATCCTGGCTA
IL-6	(F)/GAACTCCTTCTCCACAAGCG	61	272
	(R)/TTTTCTGCCAGTGCCTCTTT
ENA-78	(F)/TCTGCAAGTGTTCGCCATAG	61	363
	(R)/GCTTAAGCGCCAAACATAGG
Collagen-1α	(F)/TGGGAGTGCAAGGATACTCTATATCG	60	320
	(R)/CCCATCCCATCTTCGACGTAC
GAPDH	(F)/CCACCCATGGCAAATTCCATGGCA	60	391
	(R)/TCTAGACGGCAGGTCAGGTCCACC

^aT_m, annealing temperature; (F), forward primer; (R), reverse primer.