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. 2014 Dec 23;4:7532. doi: 10.1038/srep07532

Figure 4. Estimation of vesicle adsorption on the lumen of dialysis membranes.

Figure 4

(A, B) Colloidal Coomassie stained gel of HFDa and SDS elution of protein adsorbed on the surface of the membrane starting from 200 ml of urine, processed in 8 independent devices. Gel loading was based on the BCA assay with 10 µg of protein per lane. C and D, the respective gel as above immunostained for TSG101 and THP. Both membranes run from same experiment and at the same laser intensity set at a value to reach the limit of saturation point of the most abundant band. (TSG101: 153.69 ± 30.97 Kilo Integrated Intensity (KII), limits 190.06–138.14; CV 15.5%; THP 237.60 ± 80.09 KII, limits 332.57–127.28, CV 25.7%) and SDS dialysis membrane fraction (TSG101: 27.93 ± 11.22 KII, limits 46.78–16.05 CV 6.1%; THP 104.15 ± 62.90 KII, limits 267.05–62.56 CV 20.0%). Asterisk at 100 kDa indicates the Tamm-Horsfall Protein. Molecular weights are expressed in kilo Daltons.