FIG. 5.

Production of IFN-γ by lung cells cocultured with antigen-pulsed macrophages. Lung cells were isolated from BALB/c (solid bars) or DO11.10 TCR transgenic (open bars) mice infected for 30 or 50 days and were incubated with macrophages that were prepulsed with ovalbumin (A and C) or virulent M. tuberculosis (B and D). Culture supernatants were assayed for the presence of IFN-γ by ELISA (A and B) or by measuring nitrates by the Greiss reaction (C and D). The data are expressed as the means for four individual mice plus standard errors of the means and are representative of three independent experiments. Statistical significance comparing BALB/c and DO11.10 TCR transgenic mice at each time point was determined by the Student t test. *, P < 0.05; **, P < 0.005.