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. 2014 Dec 23;4:7589. doi: 10.1038/srep07589

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pH, temperature and ROS/RNS measurements after plasma exposure in media (A) Measurement of pH, (B) Temperature in 1 ml of RPMI media were exposed to N₂ plasma and D₂O + N₂ plasma for different time interval 1, 3, 5 and 10 min; (C) Determination of OH concentration; (D) Determination of H₂O₂ concentration; (E) NO in 1 ml media after N₂ and D₂O + N₂ plasma exposure for 1, 3, and 5 min only. All values are expressed as ± SD in triplicates. Students' t-test was performed to control (* denotes P<0.05 and ** denotes P<0.01).