TABLE 4.

T-cell responses of C3H/HeN male mice at 7 weeks postinoculation after infection with C. trachomatis MoPn in the meatus urethra^a

Group	T-cell-enriched splenocytes					Cells from iliac and caudal lymph nodes
	Proliferative response to (103 cpm ± 1 SD):		In vitro cytokine productionb to EB (pg/ml)			Proliferative response to (103 cpm ± 1 SD):		In vitro cytokine productionc to EB (pg/ml)
	EB	Medium	IFN-γ	IL-4	TNF-α	EB	Medium	IFN-γ	IL-4	TNF-α
Infected	11.4 ± 2.0e	0.3 ± 0.06	3,897 ± 514f	0.9	352 ± 3f	2.9 ± 0.3e	0.4 ± 0.1	2,167 ± 14f	<0.1	35 ± 8f
Controld	0.6 ± 0.2	0.3 ± 0.1	397 ± 15	0.6	52 ± 2	0.5 ± 0.1	0.3 ± 0.05	4 ± 9	<0.1	8 ± 5

^aResults are means of triplicate cultures. Data correspond to one of the experiments representative of duplicate experiments. UV-inactivated C. trachomatis MoPn EB were added at a 10:1 ratio to the T cells.

^bA total of 2.5 × 10⁶ T-cell-enriched splenocytes were stimulated

^cA total of 1.5 × 10⁶ cells from lymph nodes were stimulated.

^dSham infected with SPG.

^eP < 0.05, by the Mann-Whitney U test, compared to the sham-infected group.

^fP < 0.05, by Student's t test, compared to the sham-infected group.