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IgA anti-Brucella LPS antibodies in saliva obtained at three different time points from mice immunized orally with 1011 strain WR201 bacteria. Saliva was collected with a micropipette from five mice in each group after i.p. injection of 70 to 100 μg of pilocarpine. Total salivary Brucella-specific IgA levels were measured by ELISA (P = 0.0598 for dead and P = 0.01 for live WR201).
