TABLE 1.
Frequency of organ infection at various time points after oral administration of different doses of strain WR201 bacteriaa
| Day | No. of organs infected at indicated dose (CFU)/total no.
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Lung
|
Liver
|
Spleen
|
|||||||
| 109 | 1010 | 1011 | 109 | 1010 | 1011 | 109 | 1010 | 1011 | |
| 3 | 1/5 | 1/4b | 2/4b | 1/5 | 1/5 | 4/4b | 1/5 | 4/5 | 5/5 |
| 7 | 2/5 | 3/4b | 1/5 | 1/5 | 4/5 | 1/5 | 4/5 | 5/5 | 5/5 |
| 14 | 0/5 | 0/5 | 1/5 | 0/5 | 0/5 | 1/5 | 5/5 | 5/5 | 5/5 |
| 28 | 0/5 | 0/5 | 1/5 | 0/5 | 0/5 | 0/5 | 1/5 | 5/5 | 5/5 |
a
Groups of five mice were immunized orally with 109, 1010, or 1011 CFU of strain WR201. At the indicated days thereafter, mice were sacrificed and the number of bacterial CFU in organ homogenates was determined by serial dilution and plating on brucella agar. Data represent the number of organs infected as a fraction of the number of organs cultured. Limit of detection was 2 CFU/organ.
b
One organ from group could not be evaluated for infection due to culture plate contamination.