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TABLE 2.

Production of Brucella-specific serum antibody in mice inoculated orally with strain WR201 bacteriaa

WR201 dose(CFU) Antibody level (OD ± SD)
Anti-LPS
Anti-RFBL
IgG IgM IgG IgM
109 913 ± 2,008 31 ± 47 49 ± 102 1.2 ± 0.45
1010 3,069 ± 3,863 65 ± 78 47 ± 90 1 ± 0
1011 3,686 ± 2,959 87 ± 82 62 ± 87 1 ± 0
a

Mice were inoculated with 109, 1010, or 1011 CFU of WR201, and blood was collected 4 weeks later for determination of serum anti-LPS or anti-RFBL antibody levels by ELISA. OD units represent the dilution of serum required to give an A410 of 0.5.