TABLE 5.

Production of IL-2 and IFN-γ from mice immunized with live or dead strain WR201 bacteria^a

Inoculum	IL-2 (pg/ml ± SD) from cells cultured with:		IFN-γ pg/ml ± SD from cells cultured with:
	Medium	RFBL	Medium	RFBL
Saline	5 ± 4	4 ± 4	94 ± 94	208 ± 200
Dead WR201	3 ± 3	8 ± 9	276 ± 328	276 ± 333
Live WR201	4 ± 2	219 ± 180b	147 ± 131	1,607 ± 1,466c

^aMice (n = 5/group) were sham immunized with saline or with dead or live WR201. Eight weeks later, spleen cells from individual mice were cultured with medium or RFBL. Supernatant fluids were collected 48 h later and assayed by ELISA for cytokine content. Data are expressed as means ± SD.

^bSignificantly different from both dead and saline inoculum groups by ANOVA on log-normalized data with Tukey's simultaneous pairwise comparisons.

^cP = 0.056 versus dead inoculum group and P = 0.071 versus saline inoculum group by ANOVA on log-normalized data with Tukey's simultaneous pairwise comparisons.